Dimethyl sulfoxide improves sensitivity and specificity of RT-PCR and qRT-PCR amplification of low-expressed transgenes
نویسندگان
چکیده
منابع مشابه
Dimethyl Sulfoxide Improves Sensitivity and Specificity of Rt-pcr and Qrt-pcr Amplification of Low-expressed Transgenes
The expression of transgenes in a host plant may be low for a number of reasons. Both low expression and poor specificity of amplification were encountered during analysis of the expression of the Arabidopsis cytokinin oxidase/dehydrogenase (AtCKX1) gene in transgenic Centaurium erythraea. The optimization of the PCR protocol involved a gradient of annealing temperatures, as well as the applica...
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ژورنال
عنوان ژورنال: Arhiv za bioloske nauke
سال: 2012
ISSN: 0354-4664,1821-4339
DOI: 10.2298/abs1203865s